quick start bradford protein assay  (Bio-Rad)

Journal: Purinergic Signalling

Article Title: Tumor growth inhibitory activity of the P2X7 receptor antagonist AZ10606120 in two cell lines of human glioblastoma

doi: 10.1007/s11302-026-10144-8

Figure Lengend Snippet: Cell viability and protein measured in U87MG and T98G GBM cells after treatment with AZ10606120. The effects of the antagonist AZ10606120 (5–100 µM) were assessed using the XTT assay (Fig. 3 A and B ). Cell viability was expressed as % of the control. Figures 3 C and D show protein (µg/µl) measured by the Bradford method after 72 h of treatment with AZ10606120 (5–100 µM). Values represent the mean ± standard error (SEM) of three independent experiments performed in six replicates and evaluated by one-way ANOVA followed by Dunnett's post-test. (N = 3) ** p < 0.01; *** p < 0.005; **** p < 0.0001. All p -values were calculated relative to control

Article Snippet: Bradford protein assay (Quick StartTM Bradford Protein Assay Kit, Biorad, Hercules, CA, USA) was performed to quantize protein levels.

Techniques: XTT Assay, Control